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- Hexane Extract of
Orthosiphon stamineus Induces Insulin Expression and Prevents Glucotoxicity in INS-1 Cells - Hae-Jung Lee, Yoon-Jung Choi, So-Young Park, Jong-Yeon Kim, Kyu-Chang Won, Jong-Keun Son, Yong-Woon Kim
- Diabetes Metab J. 2015;39(1):51-58. Published online February 16, 2015
- DOI: https://doi.org/10.4093/dmj.2015.39.1.51
- 4,461 View
- 62 Download
- 10 Web of Science
- 8 Crossref
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Abstract
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PubReader Background Hyperglycemia, a characteristic feature of diabetes, induces glucotoxicity in pancreatic β-cells, resulting in further impairment of insulin secretion and worsening glycemic control. Thus, preservation of insulin secretory capacity is essential for the management of type 2 diabetes. In this study, we evaluated the ability of an
Orthosiphon stamineus (OS) extract to prevent glucotoxicity in insulin-producing cells.Methods We measured insulin mRNA expression and glucose-stimulated insulin secretion (GSIS) in OS-treated INS-1 cells after exposure to a high glucose (HG; 30 mM) concentration.
Results The hexane extract of OS elevated mRNA expression of insulin as well as pancreatic and duodenal homeobox-1 of INS-1 cells in a dose-dependent manner. The hexane OS extract also increased the levels of phosphorylated phosphatidylinositol 3-kinase (PI3K) in a concentration-dependent manner. Additionally, Akt phosphorylation was elevated by treatment with 100 and 200 µmol of the hexane OS extract. Three days of HG exposure suppressed insulin mRNA expression and GSIS; these expressions were restored by treatment with the hexane OS extract. HG elevated peroxide levels in the INS-1 cells. These levels were unaffected by OS treatment under both normal and hyperglycemic conditions.
Conclusion Our results suggested that the hexane extract of OS elevates insulin mRNA expression and prevents glucotoxicity induced by a 3-day treatment with HG. This was associated with the activation of PI-3K and Akt.
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Citations
Citations to this article as recorded by
- An Updated Review of Ethnobotany, Ethnopharmacology, Phytochemistry and Pharmacological Activities of Orthosiphon stamineus Benth
Anandarajagopal Kalusalingam, Dania Najiha Hasnu, Abdullah Khan, Ching Siang Tan, Bama Menon, Venkateshan Narayanan, Khang Wen Goh, Asmuni Mohd Ikmal, Noraini Talip, Poonguzhali Subramanian, Long Chiau Ming
Malaysian Applied Biology.2024; 53(1): 1. CrossRef - Scopoletin protects INS-1 pancreatic β cells from glucotoxicity by reducing oxidative stress and apoptosis
Jae Eun Park, Ji Sook Han
Toxicology in Vitro.2023; 93: 105665. CrossRef - A Systematic Review of Orthosiphon stamineus Benth. in the Treatment of Diabetes and Its Complications
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Molecules.2022; 27(2): 444. CrossRef - Comprehensive chemical and metabolic profiling of anti‐hyperglycemic active fraction from Clerodendranthi Spicati Herba
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Journal of Separation Science.2021; 44(9): 1805. CrossRef - Short-Term Protocols to Obtain Insulin-Producing Cells from Rat Adipose Tissue: Signaling Pathways and In Vivo Effect
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Plant Methods.2019;[Epub] CrossRef - 50% Ethanol extract of Orthosiphon stamineus modulates genotoxicity and clastogenicity induced by mitomycin C
Dhamraa Waleed Al-dualimi, Aman Shah Abdul Majid, Sarah Furqan Faisal Al-Shimary, Amal Aziz Al-Saadi, Raghdaa Al Zarzour, Muhammad Asif, Chern Ein Oon, Amin Malik Shah Abdul Majid
Drug and Chemical Toxicology.2018; 41(1): 82. CrossRef - Can Tea Extracts Exert a Protective Effect Against Diabetes by Reducing Oxidative Stress and Decreasing Glucotoxicity in Pancreatic β-Cells?
Heeyoung Chae, Patrick Gilon
Diabetes & Metabolism Journal.2015; 39(1): 27. CrossRef
- An Updated Review of Ethnobotany, Ethnopharmacology, Phytochemistry and Pharmacological Activities of Orthosiphon stamineus Benth
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